Yes, red circled hose connection should be fuel tap vacuum. There's no other sane usage of that hose.
To be honest, i can only guess. All carbs I've seen yet had only two connections. One on top end (red) for fuel fumes and other on bottom end near needle valve (pink). I have no idea what is purpose of yellow hoses.
Despite that both your and mine carbs are Mikuni, they are different. But, I still have one theory about them. In the photo, you can see airbox side of B4 Mikuni carb with slide and slide holder both removed, so you can see throttle flap too which is normaly obscured by slide. You can see that upper part of intake manifold has thin "submanifold". This one is for supply of reference pressure for slider diaphragm. You can see, that manifolds of B250 carbs are missing this "submanifold". Therefore I think that:
* larger red holes are for reference pressure for slider diaphragm. You can test this theory by applying some slight air pressure to this hole (just blow there stronger) and one or both sliders of interconnected carbs should rise slightly. Look at the air box, if you can find some holes which may fit these one. Normally B4 air box contains only one hole for crankcase ventilation. You air box will either contain more than one hole, or there was T- or X- piece connecting red holes with air box with crankcase ventilation and air box throught connection piece.
* smaller yellow holes are fuel fumes hoses. You can test this too by blowing in there. If carbs are in normal upward position, you should feel some air leaking from pink bottom hole (some will also leak from other holes in carbs). If you position carbs upside down, no (or much much less) air should be leaking from pink hole
* smaller pink hole is for fuel intake. Test process is the opposite as for fuel fumes holes.
Distinction between fuel fume and intake is easy. Fumes naturally goes up, so upper of pair should be for fumes and latter should be for intake.
About that coolant line, to be honest, I didn't examine it so deeply. I just figured that I have T-piece on this overflow line and third hose looks like it is connected to bottom of fuel tank. I can look at this today if I be in garage and hopefully B4 has same piping like B250.